实验室动态

中国科学院水生生物研究所藻类细胞生物学实验室

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    journal club


    7月28日,细胞生物学组进行题为“Interaction of heterotrimeric kinesin-II with IFT-B–connecting tetramer is crucial for ciliogenesis”的journal club。


    ABSTRACT

    Intraflagellar transport (IFT) is crucial for the assembly and maintenance of cilia and is mediated by IFT particles containing IFT-A and IFT-B complexes. IFT-B powered by heterotrimeric kinesin-II and IFT-A powered by the dynein-2 complex are responsible for anterograde and retrograde protein trafcking, respectively. However, little is known about the molecular basis of the trafcking of these IFT particles regulated by kinesin and dynein motors. Using the visible immunoprecipitation assay, we identifed in this study a three-to-four protein interaction involving the kinesin-II trimer KIF3A–KIF3B–KAP3 and the IFT-B–connecting tetramer IFT38–IFT52–IFT57–IFT88; among the kinesin-II subunits, KIF3B contributed mainly to IFT-B binding. Furthermore, we showed that the ciliogenesis defect of KIF3B-knockout cells can be rescued by the exogenous expression of wild-type KIF3B but not by that of its mutant compromised with respect to IFT-B binding. Tus, interaction of heterotrimeric kinesin-II with the IFT-B–connecting tetramer is crucial for ciliogenesis via the powering of IFT particles to move in the anterograde direction.


    Interaction of heterotrimeric kinesin-II with the IFT-B–connecting tetramer