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中国科学院水生生物研究所藻类细胞生物学实验室

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    journal club


    5月19日,油滴组进行题为“Comprehensive Discovery of Cell-cycle-essential Pathways in Chlamydomonas reinhardtii”的 journal club。


    ABSTRACT

    We generated a large collection of temperature-sensitive lethal mutants in the unicellular green alga Chlamydomonas reinhardtii, focusing on mutations specifically affecting cell cycle regulation. We used UV mutagenesis and robotically assisted phenotypic screening to isolate candidates. To overcome the bottleneck at the critical step of molecular identification of the causative mutation (‘driver’), we developed MAPS-SEQ (Meiosis-Assisted Purifying Selection Sequencing), a multiplexed genetic/bioinformatics strategy. MAPS-SEQ allowed us to perform multiplexed simultaneous determination of the driver mutations from hundreds of neutral ‘passenger’ mutations in each member of a large pool of mutants. This method should work broadly, including in multicellular diploid genetic systems, for any scorable trait. Using MAPS-SEQ, we identified essential genes spanning a wide range of molecular functions. Phenotypic clustering based on DNA content analysis and cell morphology indicated that the mutated genes function in the cell cycle at multiple points and by diverse mechanisms. The collection is sufficiently complete to allow specific conditional inactivation of almost all cell-cycle-regulatory pathways. ~75% of the essential genes identified in this project had clear orthologs in land plant genomes, a huge enrichment compared to the value of ~20% for the Chlamydomonas genome overall. Findings about these mutants will likely have direct relevance to essential cell biology in land plants.