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中国科学院水生生物研究所藻类细胞生物学实验室

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    journal club


    11月18日,油滴组进行题为“A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii”的journal club。



    Fig. 1. Construct for testing the potential activity of an sgRNA gene located within an artificial intron of a Cas9 gene (i.e., a gene-within-a-gene).


    Fig. 2. DNA sequence confirmation of Cas9/intron-sgRNA-directed mutagenesis of the sgRNA target site within the Chlamydomonas FKB12 gene of cells transformed with a Cas9/intronsgRNA construct.


    Fig. 3. Gene editing by homologous recombination or nucleotide replacement in the non-functional argininosuccinate lyase (ARG) gene (Cre01.g021251.t1.1 - (M = 1) 4.3.2.1) of

    Chlamydomonas, cw 15 arg7-8 mt+ (cc4350).


    Fig. 4. Gene editing by homologous recombination or nucleotide replacement in the Chlamydomonas acetolactate synthase (ALS) gene (Cre09.g386758.t1.1).