实验室动态

中国科学院水生生物研究所藻类细胞生物学实验室

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地址:湖北省武汉市武昌区东湖南路7号中科院水生所2号楼816

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    journal club


    10月27日,课题组进行题为“Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zincfinger Nucleases and CRISPR/Cas9”的journal club。

    Figure 1. Chlamydomonas Photoreceptors

    Figure 2. Optimization of zinc fnger nuclease (ZFNs) and transformation parameters

    Figure 3. Inactivation of non-selectable genes using zinc-fnger nucleases

    Figure 5. Gene disruption by genetically encoded Cas9

    Figure 6. Gene targeting using exogenously supplied recombinant Cas9/gRNA ribonucleoprotein (RNP) complexes

    Figure 7. Protocols for gene editing in Chlamydomonas reinhardtii