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中国科学院水生生物研究所藻类细胞生物学实验室

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    journal club


      9月16日,油滴组进行题为“CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii”的journal club

    Figure 1. Map of CRISPR/Cas9 target sites in the MAA7, CpSRP43 and ChlM loci, and in vitro cleavage of target DNAs by sgRNAs and the Cas9 protein.


        

    Figure 2. Auxotrophic selection of MAA7 mutants and their sequence analyses.


    Figure 3. Complementation of MAA7 mutants with WT and MAA7 20-1 TSB sequences.


    Figure 4. Phenotypic characterization of CpSRP43 mutants.


    Figure 5. NHEJ-mediated knock-in of the co-transformed vector at the CRISPR/Cas9 cut site in the CpSRP43 locus.


    Figure 6. Phenotypic characterization of light green mutants induced by CRISPR/Cas9 at the ChlM locus.

      

    Figure 7. NHEJ-mediated knock-in mutants of the co-transformed vector at the CRISPR/Cas9 cut site on the ChlM locus.